Record: 48-49-3, .495 W-L% (7th of 12) (Schedule and Results)
SRS: 10.05 (2nd of 12) ▪ SOS: 10.08 (2nd of 12)
Conference Champion: Michigan State
Ranked in Preseason AP Poll: 3 (Michigan, Ohio State and Purdue)
Ranked in Final AP Poll: 1 (Michigan State)
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
|---|---|---|---|---|---|---|---|---|
| 1 | Sep 25, 1965 | Sat | Northwestern | 20 | @ | Indiana | 0 | |
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 2 | Oct 2, 1965 | Sat | (9) Michigan State | 22 | Illinois | 12 | ||
| 3 | Oct 2, 1965 | Sat | Wisconsin | 16 | Iowa | 13 | ||
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 4 | Oct 9, 1965 | Sat | (5) Michigan State | 24 | @ | Michigan | 7 | |
| 5 | Oct 9, 1965 | Sat | Minnesota | 42 | Indiana | 18 | ||
| 6 | Oct 9, 1965 | Sat | Ohio State | 28 | Illinois | 14 | ||
| 7 | Oct 9, 1965 | Sat | (6) Purdue | 17 | @ | Iowa | 14 | |
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 8 | Oct 16, 1965 | Sat | Illinois | 34 | Indiana | 13 | ||
| 9 | Oct 16, 1965 | Sat | (4) Michigan State | 32 | Ohio State | 7 | ||
| 10 | Oct 16, 1965 | Sat | Minnesota | 14 | @ | Iowa | 3 | |
| 11 | Oct 16, 1965 | Sat | (7) Purdue | 17 | @ | Michigan | 15 | |
| 12 | Oct 16, 1965 | Sat | Wisconsin | 21 | @ | Northwestern | 7 | |
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 13 | Oct 23, 1965 | Sat | (2) Michigan State | 14 | @ | (6) Purdue | 10 | |
| 14 | Oct 23, 1965 | Sat | Minnesota | 14 | Michigan | 13 | ||
| 15 | Oct 23, 1965 | Sat | Northwestern | 9 | Iowa | 0 | ||
| 16 | Oct 23, 1965 | Sat | Ohio State | 20 | @ | Wisconsin | 10 | |
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 17 | Oct 30, 1965 | Sat | Illinois | 21 | (6) Purdue | 0 | ||
| 18 | Oct 30, 1965 | Sat | Indiana | 21 | Iowa | 17 | ||
| 19 | Oct 30, 1965 | Sat | Michigan | 50 | Wisconsin | 14 | ||
| 20 | Oct 30, 1965 | Sat | (1) Michigan State | 49 | Northwestern | 7 | ||
| 21 | Oct 30, 1965 | Sat | Ohio State | 11 | Minnesota | 10 | ||
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 22 | Nov 6, 1965 | Sat | Michigan | 23 | @ | Illinois | 3 | |
| 23 | Nov 6, 1965 | Sat | (1) Michigan State | 35 | @ | Iowa | 0 | |
| 24 | Nov 6, 1965 | Sat | Minnesota | 27 | Northwestern | 22 | ||
| 25 | Nov 6, 1965 | Sat | Ohio State | 17 | Indiana | 10 | ||
| 26 | Nov 6, 1965 | Sat | Purdue | 45 | Wisconsin | 7 | ||
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 27 | Nov 13, 1965 | Sat | Illinois | 51 | @ | Wisconsin | 0 | |
| 28 | Nov 13, 1965 | Sat | (1) Michigan State | 27 | Indiana | 13 | ||
| 29 | Nov 13, 1965 | Sat | Northwestern | 34 | Michigan | 22 | ||
| 30 | Nov 13, 1965 | Sat | Ohio State | 38 | Iowa | 0 | ||
| 31 | Nov 13, 1965 | Sat | Purdue | 35 | Minnesota | 0 | ||
| Rk | Date | Day | Winner/Tie | Pts | Loser/Tie | Pts | Notes | |
| 32 | Nov 20, 1965 | Sat | Illinois | 20 | @ | Northwestern | 6 | |
| 33 | Nov 20, 1965 | Sat | Minnesota | 42 | Wisconsin | 7 | ||
| 34 | Nov 20, 1965 | Sat | Ohio State | 9 | @ | Michigan | 7 | |
| 35 | Nov 20, 1965 | Sat | Purdue | 26 | @ | Indiana | 21 |